Extracting RNA from the seeds was never easy as mentioned by one of the PostDocs of the International Rice Research Institute(IRRI). As a researcher of IRRI, I've worked with those samples close to three years and indeed STARCH is a big problem in the RNA extraction. Face with this problem, I developed or optimised a protocol on how to extract the RNA with the LEAST amount of STARCH possible. By the way, starch causes one of the major problem in downstream applications like conventional PCR and especially real time PCR. One of the procedures, I emphasized is the used of doubly repeated LiCl precipitation. If you want some advice or if you want a copy of the protocol, please feel free to email me at magponcio@gmail.com.
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